Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 183, Issue 2, Pages 488-502Publisher
HUMANA PRESS INC
DOI: 10.1007/s12010-017-2569-2
Keywords
Biogas upgrade; Biomethane; Hydrogenotrophic methanogens; Biotrickling filter
Funding
- National Science Foundation's Partnership for International Research & Education (PIRE) grant [1243433]
- Office Of The Director
- Office Of Internatl Science &Engineering [1243433] Funding Source: National Science Foundation
Ask authors/readers for more resources
This research reports the development of a biotrickling filter (BTF) to upgrade biogas, which is achieved by adding H-2 to reduce CO2. H-2 and CO2 (80:20% vol.) were fed to a bench-scale BTF packed with polyurethane foam (PUF) and inoculated with hydrogenotrophic methanogens. Maximum CH4 production rates recorded were as high as 38 m(3) (CH4) m(-3) (reactor) day(-1), which is 5-30 times faster than earlier reports with other kinds of bioreactors. The high rates were attributed to the efficient mass transfer and high density of methanogens in the BTF. The removal efficiencies for H-2 and CO2 were 83 and 96%, respectively. 5-Cyano-2,3-ditolyl tetrazolium chloride/DAPI staining revealed that 67% of cells were alive near the gas entrance port, while only 8.3% were alive at the exit. Furthermore, DNA sequencing showed that only 27% of the biomass was composed of Euryarchaeota, the phylum which includes methanogens. These two observations suggest that optimizing the methanogen density and activity could possibly reach even higher biogas upgrading rates.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available