Journal
VACCINE
Volume 35, Issue 1, Pages 1-9Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2016.11.063
Keywords
LCMV; SIV; NHP
Categories
Funding
- NIH [AI007245, AI07387, AI078526, AI096040]
- Bill and Melinda Gates Foundation [OPP1033091]
- Ragon Institute of MGH, MIT, and Harvard
- Herchel Smith Graduate Fellowship
- Hookipa Biotech
- Austrian Research Promotion Agency (Die Osterreichische Forschungsforderungsgesellschaft, FFG)
- Bill and Melinda Gates Foundation [OPP1033091] Funding Source: Bill and Melinda Gates Foundation
Ask authors/readers for more resources
An important focus in vaccine research is the design of vaccine vectors with low seroprevalence and high immunogenicity. Replication-incompetent lymphocytic choriomeningitis virus (rLCMV) vectors do not elicit vector-neutralizing antibody responses, and homologous prime-boost regimens with rLCMV vectors induce boostable and protective T cell responses to model antigens in mice. However, cellular and humoral immune responses following homologous rLCMV vaccine regimens have not been rigorously evaluated in non-human primates (NHPs). To test whether rLCMV vectors constitute an effective vaccine platform in NHPs, we developed rLCMV vectors expressing SIVmac239 Env and Gag antigens and assessed their immunogenicity in mice and cynomolgus macaques. Immunization with rLCMV vaccine vectors expressing SIV Env and Gag was effective at generating Sly-specific T cell and antibody responses in both mice and NHPs. Epitope mapping using SIN/ Env in C57BL/6 mice demonstrated that rLCMV vectors induced sustained poly-functional responses to both dominant and subdominant epitopes. Our results suggest the potential of rLCMV vectors as vaccine candidates. Future SIV challenge experiments in rhesus macaques will be needed to assess immune protection by these vaccine vectors. (C) 2016 The Authors. Published by Elsevier Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available