Journal
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Volume 58, Issue 12, Pages 5242-5250Publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.17-22479
Keywords
polypoidal choroidal vasculopathy; immunology; microglia; blood; monocytes
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Funding
- Danish Eye Research Foundation (Taastrup, Denmark)
- Fight for Sight Denmark (Copenhagen, Denmark)
- Velux Foundation (Soborg, Denmark)
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PURPOSE. To investigate surface expression of CD11b and CD200 on circulating monocytes in patients with polypoidal choroidal vasculopathy (PCV). METHODS. This was a prospective case-control study of patients with PCV (n = 27), agematched healthy controls (n = 27), and patients with neovascular AMD (n 49). All participants underwent a comprehensive ocular examination. Fluorescein and indocyanine green angiography were performed in patients suspected of neovascular AMD or PCV Polypoidal choroidal vasculopathy was angiographically categorized into those with a strong presence of a branching vascular network (BVN) (type 1) or with a faint/no clear presence of a BVN (type 2). Fresh venous blood was stained with fluorescent antibodies for flow cytometric analyses. We compared the percentages of CD11b(+), CD200(+), and CD11b(+) CD200(+) monocytes between groups of diagnosis and between different angiographic subtypes of PCV. RESULTS. Overall, CD11b(+) monocytes were both increased in patients with PCV and neovascular AMD. CD200(+) and CD11b(+) CD200(+) monocytes were increased in patients with neovascular AMD. An age-related increase in CD11b(+) CD200(+) monocytes was absent in patients with PCV and neovascular AMD. Patients with PCV type 1 had significantly higher CD11b(+), CD200(+), and CD11b(+) CD200(+) monocytes, whereas patients with PCV type 2 had levels similar to that in healthy controls. CONCLUSIONS. We found that PCV is immunologically heterogeneous with significant differences between angiographic subtypes. Increased CD11b(+) and CD200(+) monocytes in those with a strong presence of BVN indicate that BVN development may be associated with retinal injury and a VEGF-mediated process that is either reflected or propelled by systemic changes in monocytes.
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