Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 22, Pages 12152-12159Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b02791
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Funding
- National Basic Research Program of China (973 Program) [2013CB934400, 2012CB932400]
- National Natural Science Foundation of China [61361160412, 31400860, 21575096, 21605109]
- Collaborative Innovation Center of Suzhou Nano Science and Technology
- '111' program from the Ministry of Education (MOE) of China
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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Long-term and real-time investigation of the dynamic process of changes is critically significant for understanding the related pathogenesis of diseases and the design of intracellular drug delivery systems. Herein, we present a one-step synthetic strategy to construct ratiometric pH sensors, which are made of europium (Eu)-doped one-dimensional silicon nanorods (Eu@SiNRs). The as-prepared Eu@SiNRs have distinct emission maxima peaks at 470 and 620 nm under 405 nm excitation. Of particular note, the fluorescence emission intensity at 470 nm decreases along with the increase of pH, while the one at 620 nm is nearly unaffected by pH changes, making Eu@SiNRs a feasible probe for pH sensing ratiometrically. Moreover, Eu@SiNRs are found to be responsive to a broad pH range (ca. 3-9), biocompatible (e.g., similar to 100% of cell viability during 24 h treatment) and photostable (e.g., similar to 10% loss of intensity after 40 min continuous UV irradiation). Taking advantages of these merits, we employ Eu@SiNRs for the visualization of the cytoplasmic alkalization process mediated by nigericin in living cells, for around 30 min without interruption, revealing important information for understanding the dynamic process of pH(i) fluctuations.
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