Journal
BIOTECHNOLOGY LETTERS
Volume 39, Issue 12, Pages 1811-1819Publisher
SPRINGER
DOI: 10.1007/s10529-017-2422-2
Keywords
Fast glycolytic fibers; Heterozygous myostatin knockout; Myosin heavy chain isoforms; Myostatin; Somatic cell nuclear transfer; Transcription activator-like effector nuclease
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Funding
- Science and Technology Development Projects of Jilin Province of China [20160520057JH]
- ToolGen, Inc.
- Hanji and Longxing pig farms
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To explore the effects of heterozygous myostatin-knockout (MSNT+/-) on muscle characteristics, specifically fiber-type distribution and expression of myosin heavy chain isoforms in pigs. The fiber cross-sectional area of the semitendinosus and semimembranosus muscles were much larger in MSTN+/- pigs at birth than in wild-type (WT) pigs. MSTN+/- pigs had a higher proportion of fast-type fibers and lower succinate dehydrogenase activity in muscles than WT pigs. The myosin heavy chain IIB mRNA level in both two muscles was similar to threefold higher in MSTN+/- pigs compared with WT pigs. MSTN+/- pigs exhibit a disproportionate increase in muscle mass and can have a higher body weight due to fiber hypertrophy, a change in the fiber-type distribution, and alteration of myosin heavy chain isoforms levels, leading to more fast glycolytic fibers.
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