Journal
ACS OMEGA
Volume 2, Issue 12, Pages 8843-8848Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.7b01405
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Funding
- Ministry of Education, Science, Sports, Culture and Technology (Japan) [26288083, 16K17938]
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [17H03091, 26288083, 16K17938] Funding Source: KAKEN
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In this study, we demonstrated that F-19 NMR can be used to study the thrombin-binding aptamer (TBA) DNA G-quadruplex, widely used as a model structure for studying G-quadruplex aptamers. We systematically examined the structural feature of the TBA G-quadruplex aptamer with fluorine-19 (F-19) labels at all of the thymidine positions. We successfully observed the structural change between the G-quadruplex and the unstructured single strand by F-19 NMR spectroscopy. The thermodynamic parameters of these DNA G-quadruplex aptamers were also determined from the F-19 NMR signals. We further showed that the F-19 NMR method can be used to observe the complex formed by TBA G-quadruplex and thrombin. Our results suggest that F-19 NMR spectroscopy is a useful approach to study the aptamer G-quadruplex structure.
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