Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 77, Issue -, Pages 138-140Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2017.07.020
Keywords
Antiviral capacity; BmCPV; Resistance; RNAi; Silkworm; Transgenic
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Funding
- National Natural Science Foundation of China [31501875]
- Basic Science and Frontier Technology Research of Chongqing [cstc2016jcyjA0524]
- Fundamental Research Funds for the Central Universities [XDJK2016C010, SWU115048]
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Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), a major pathogen of silkworms, causes serious economic losses in sericulture. The BmCPV genome contains 10 discrete dsRNA segments; among these, S1, S2, S3, S4, S6, and S7 encode virus structural proteins, whereas S5, 58, S9, and SW encode nonstructural proteins. In an attempt to create an anti-BmCPV silkworm strain, we constructed trans genic RNAi vector pb-CNS for knockdown of S5, S8, S9, and S10, and pb-SNS targeting S1, S2, S4, S5, and S8. Transgenic silkworm line CNS and SNS were generated via microinjection of the practical diapause silkworm strain Furong. Following infection via the oral administration of a high dose of BmCPV, the mortality rates of the nontransgenic control, CNS, and SNS were 91%, 37%, and 41%, respectively. qPCR showed that the viral mRNA content in CNS and SNS was significantly lower than that in the nontransgenic line. The economic traits of CNS and SNS were not affected. These results suggest that the knockdown of multiple BmCPV genes significantly enhances the antiviral capacity of the silkworm. (C) 2017 Published by Elsevier Ltd.
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