Journal
BIORESOURCE TECHNOLOGY
Volume 245, Issue -, Pages 743-750Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.08.194
Keywords
Biochar; Carbon dioxide sequestration; Immobilization; Lipid; Serratia sp ISTD04; Transesterification
Funding
- Department of Biotechnology (DBT), Government of India
- IMobS3 Laboratory of Excellence [ANR-10-LABX-16-01]
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An extracellular lipase was purified and characterized from psychrotolerant bacterium Pseudomonas sp. ISTPL3 isolated from Pangong lake. Lipase was purified by sequential methods of ammonium sulphate precipitation, dialysis, DEAE-cellulose ion exchange chromatography and Sephadex G-100 gel filtration chromatography, resulting in a purification fold of 6.53 and yield of 5.45%. The molecular weight was approximately 31 kDa. The purified lipase was used for transesterification of lipids produced by oleaginous chemolithotrophic bacterium Serratia sp. ISTD04 for production of biodiesel. Upon biochemical characterization, lipase was found to be alkalophilc, thermostable, active in organic polar solvents and sensitive to detergents. Further, lipase was immobilized on activated biochar to assess its transesterification efficiency during biodiesel production. Immobilized lipase gave the highest yield of fatty acid methyl esters (FAMEs) (92.23%) > unimmobilized lipase > NaOH. The immobilized lipase was assessed for its reusability and retained 75.11% of its activity after 3 cycles of biodiesel production.
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