Journal
CURRENT OPINION IN BIOMEDICAL ENGINEERING
Volume 4, Issue -, Pages 32-39Publisher
ELSEVIER
DOI: 10.1016/j.cobme.2017.09.004
Keywords
Multiphoton microscopy fluorescence imaging; Scattering and absorption in brain tissue; Laser sources for multiphoton microscopy; Fluorophores for multiphoton microscopy
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Funding
- National Institutes of Health [NS082518, NS078791, EB011556]
- American Heart Association [14EIA18970041]
- Cancer Prevention and Research Institute of Texas [RR160005]
- National Science Foundation Graduate Research Fellowship Program [DGE-1110007]
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We present a review of imaging deep-tissue structures with multiphoton microscopy. We examine the effects of light scattering and absorption due to the optical properties of biological sample and identify 1300 nm and 1700 nm as ideal excitation wavelengths. We summarize the availability of fluorophores for multiphoton microscopy as well as ultrafast laser sources to excite available fluorophores. Lastly, we discuss the applications of multiphoton microscopy for neuroscience.
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