Journal
TRENDS IN PLANT SCIENCE
Volume 22, Issue 1, Pages 38-52Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tplants.2016.08.009
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Funding
- Spanish Ministry of Economy and Competitiveness [B102014-54426-P, B102014-54441-P]
- Catalan Government - Agricultural Biotechnology Research [2014 SGR 1296]
- European Research Council Advanced Grant 'Future-Pharma' [269110]
- Dow AgroSciences for research on zinc -finger nucleases
- ICREA Funding Source: Custom
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Designer nucleases allow the creation of new plant genotypes by introducing precisely-targeted double-strand breaks that are resolved by endogenous repair pathways. The major nuclease technologies are meganucleases, zinc finger nucleases, transcription activator-like effector nucleases, and the CRISPR/Cas9 system. Each comprises a promiscuous endonuclease guided by protein-DNA or RNA-DNA interactions. A great deal is known about the principles of designer nucleases but much remains to be learned about their detailed behavioral characteristics in different plant species. The outcome of genome engineering reflects the intrinsic properties of each nuclease and target genome, causing variations in efficiency, accuracy, and mutation structure. In this article, we critically discuss the activities of designer nucleases in different cereals representing a broad range of genome characteristics.
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