Journal
AGING-US
Volume 9, Issue 12, Pages 2471-2479Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/aging.101331
Keywords
Hirschsprung's disease; microRNA (miRNA); proliferation; migration; autophagy
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Funding
- Natural Science Foundation of China [NSFC 81370473, NSFC 81400574, NSFC 81570467]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Background: Hirschsprung's disease (HSCR) is a common digestive disease caused by impaired development of neural crest cells. Some studies have revealed the roles of microRNA (miRNA) in various diseases. But the function of miRNA in HSCR needs further investigation. Methods: We adopted qRT-PCR and immunoblot analyses to explore the relative expression of miR-939 and LRSAM1 in 80 HSCR bowel tissues and 80 normal bowel tissues. CCK-8 assay, transwell assay and flow cytometry were used to evaluate the function of miR-939 by overexpression of miR-939 in 293T, SK-N-BE(2), SHSY5Y cell lines. The direct connection between miR-939 and LRSAM1 was validated by dual-luciferase reporter assay. We also investigated the autophagy level via immunoblot analyses. Results: Mir-939 was significantly upregulated in HSCR tissues with decreased expression of LRSAM1. Overexpression of miR-939 suppressed cell proliferation without affecting cell apoptosis, cell cycle or cell migration. And LRSAM1 exerted similar function. Autophagy was impaired in HSCR tissues compared with control samples. Mir-939 did not inhibit the autophagy although it decreased the expression of LRSAM1. Conclusions: Our study shows the potential function of mir-939 through regulating LRSAM1 in HSCR and infers that autophagy may also confer the risk of HSCR.
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