Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1859, Issue 12, Pages 2289-2296Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2017.08.015
Keywords
Tear film; Tear film lipid layer; Lysozyme; Molecular dynamics; Lipid-protein interaction
Categories
Funding
- Czech Science Foundation [15-14292S]
- National Grid Infrastructure MetaCentrum (Projects of Projects of Large Research, Development, and Innovations Infrastructures) [CESNET LM2015042]
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The tear film is a thin multilayered structure covering the cornea. Its outermost layer is a lipid film underneath of which resides on an aqueous layer. This tear film lipid layer (TFLL) is itself a complex structure, formed by both polar and nonpolar lipids. It was recently suggested that due to tear film dynamics, TFLL contains in homogeneities in the form of polar lipid aggregates. The aqueous phase of tear film contains lachrymal-origin proteins, whereby lysozyme is the most abundant. These proteins can alter TFLL properties, mainly by reducing its surface tension. However, a detailed nature of protein-lipid interactions in tear film is not known. We investigate the interactions of lysozyme with TFLL in molecular details by employing coarse-grained molecular dynamics simulations. We demonstrate that lysozyme, due to lateral restructuring of TFLL, is able to penetrate the tear lipid film embedded in inverse micellar aggregates.
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