Isothermal Amplification Strategies for Detection in Microfluidic Devices

Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen-antibody recognition, has been miniaturized for micro fluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection.