Journal
GENOMICS DATA
Volume 13, Issue -, Pages 44-45Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gdata.2017.07.002
Keywords
Lung; Transcriptome; Microhyla fissipes; Unigenes
Categories
Funding
- Important Research Project of Chinese Academy of Sciences [KJZG-EW-L13]
- Western Light Talent Culture Project of the Chinese Academy of Sciences [Y6C3021]
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Microhyla fissipes, belonging to Neobatrachia, is a new model organism to study developmental biology, adaptive mechanisms from aquatic to terrestrial life, environmental toxicology, and human disease. M. fissipes use of lungs soon after hatching makes it extremely valuable for the study of lung function and development mechanisms. However, our knowledge of genes and pathways associated with lung development in M. fissipes is very limited. In this study, we conducted de novo transcriptome assembly for the lung of M. fissipes using the Illumina HiSeq4000 platform. We obtained approximately 9.0 GB clean data from the lung of the stage 28 tadpole with lung inflation (NCBI accession numbers: SRP107055). De novo transcriptome assembly identified 209,358 transcripts and 93,813 unigenes. In addition, BLASTX against NR, NT, KO, SwissProt, PFAM, GO and KOG databases were used to annotate all the 93,813 unigenes. This study provides the transcriptome and functional annotation of genes in M. fissipes lung development, which will be useful for comparative transcriptome analyses and promote research into mechanism of lung development in anuran.
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