Journal
CANCER LETTERS
Volume 410, Issue -, Pages 92-99Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2017.09.014
Keywords
Colorectal cancer; Next-generation sequencing; Tumor cellularity; Tumor heterogeneity; Chemotherapy
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Funding
- Cancer Hospital, CAMS, Beijing
- Natural Basic Research Program of China [2015M020408]
- National Natural Science Foundation of China [81702436]
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Next-generation sequencing (NGS) has been used in mutation detection of colorectal cancer (CRC). We here interrogated 747 CRC samples to detect mutations in 22 cancer-related genes by using NGS, and to explore some key challenges related to tumor biology. RAS mutations (KRAS or NRAS mutations), RAS/BRAF/PIK3CA mutations (mutations in KRAS, NRAS, BRAF or PIK3CA) and mutation burden (mutations in any of the 22 detected genes) were observed in 53.0% (396/747), 57.1% (431/747) and 84.2% (629/747) of specimens, respectively. Higher mutation frequencies were observed in biopsy specimens with >= 20% tumor cellularity than those with < 20% tumor cellularity, but these differences were not observed in resection samples. Intratumor mutational heterogeneity was estimated by mutant allele frequency and tumor cellularity, and more likely to occur in PIK3CA mutant tumors. No significant differences of mutation frequencies were detected between primary and metastatic tumors. Additionally, specimens after chemotherapy showed lower mutation frequencies compared with specimens without chemotherapy. Together, our findings demonstrate that poor tumor cellularity, tumor heterogeneity and adjuvant therapy may confound the molecular diagnosis of CRC, and should be highlighted with prospective quality assessment during tissue process. (C) 2017 Elsevier B.V. All rights reserved.
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