Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 494, Issue 3-4, Pages 575-580Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2017.09.102
Keywords
UHRF2; PIP-box; PCNA; Structure; Molecular mechanism
Categories
Funding
- Chinese Academy of Sciences [XDB 08010101]
- National Key Research and Development Program of China [2016YFA0400903]
- Foundation for Innovative Research Groups of the National Natural Science Foundation of China [31621002]
- National Natural Science Foundation of China [U1532109, 31370756, 31361163002, 31700671, 31400627]
- Scientific Research Grant of Hefei Science Center of CAS [2015SRG-HSC043, 2015HSC-UP019]
- Anhui Provincial Natural Science Foundation [1608085QC52]
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UHRF2 (Ubiquitin-like with PHD and ring finger domains 2) is an E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications and cell cycle regulation by interacting with multiple epigenetic or cell-cycle related proteins. Previous studied have identified PCNA (Proliferating cell nuclear antigen) as an interacting partner of UHRF2 by using the antibody microarray. However, the molecular mechanism and the function of UHRF2-PCNA interaction remains unclear. Here, we report the complex structure of PCNA and the peptide ((784)NEILQTLLDLFFPGYSK(800)) derived from UHRF2 that contains a PIP box. Structural analysis combined with mutagenesis experiments provide the molecular basis for the recognition of UHRF2 by PCNA via PIP-box. (C) 2017 Elsevier Inc. All rights reserved.
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