Journal
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
Volume 41, Issue 12, Pages 2093-2099Publisher
WILEY
DOI: 10.1111/acer.13509
Keywords
Alcohol; Cigarette Smoke; Adduct; Macrophage; IgA
Categories
Funding
- Clinical Resource for Lung Alcohol Investigators (NIAAA) [R24 AA019661]
- NIAAA [R01 AA008769, R01 AA017663]
- Nebraska Cancer and Smoking Disease Research Program [2017-64]
- Department of Veterans Affairs [IK6 BX003781]
- [VA I01 BX003635]
- VA [1047981, 5I01BX003635-02] Funding Source: Federal RePORTER
Ask authors/readers for more resources
BackgroundMalondialdehyde (MDA) and acetaldehyde (AA) exist following ethanol metabolism and tobacco pyrolysis. As such, lungs of individuals with alcohol use disorders (AUDs) are a target for the effects of combined alcohol and cigarette smoke metabolites. MDA and AA form a stable protein adduct, malondialdehyde-acetaldehyde (MAA) adduct, known to be immunogenic, profibrotic, and proinflammatory. MAA adduct is the dominant epitope in anti-MAA antibody formation. We hypothesized that MAA-adducted protein forms in lungs of those who both abuse alcohol and smoke cigarettes, and that this would be associated with systemically elevated anti-MAA antibodies. MethodsFour groups were established: AUD subjects who smoked cigarettes (+AUD/+smoke), smokers without AUD (-AUD/+smoke), AUD without smoke (+AUD/-smoke), and non-AUD/nonsmokers (-AUD/-smoke). ResultsWe observed a significant increase in MAA adducts in lung cells of +AUD/+smoke versus -AUD/-smoke. No significant increase in MAA adducts was observed in -AUD/+smoke or in +AUD/-smoke compared to -AUD/-smoke. Serum from +AUD/+smoke had significantly increased levels of circulating anti-MAA IgA antibodies. After 1week of alcohol that MAA-adducted protein is formed in the lungs of those who smoke cigarettes and abuse alcohol, leading to a subsequent increase in serum IgA antibodies. ConclusionsMAA-adducted proteins could play a role in pneumonia and other diseases of the lung in the setting of AUD and smoking.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available