Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 49, Pages 42612-42621Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b14836
Keywords
nanographene oxide; SPECT; MR; molecular imaging; lymph node imaging
Funding
- National Natural Science Foundation of China [11275050, 21771041, 81401514]
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PEGylated ultrasmall nanographene oxide (usNGO-PEG) has exhibited a great potential in nano-theranostics due to its newly discovered physicochemical properties derived from the rich functional groups and bonds. Herein, we developed a general, simple, and kitlike preparation approach for (MTc)-M-9m-andGd-anchored NGO-PEG using a chelator-free strategy. In this strategy, [(99m)Tcl(CO)(3)(OH2)(3)]+(abbreviated to (TcI)-Tc-99m) and GdCl3 were mixed with usNGO-PEG to yield Tc-99m- and Gd-usNGO-PEG via the synergistic coordination of N and 0 atoms from NGO and PEG with (99m)Tcl and Gd3+ without additional exogenous chelators. Under optimized conditions, the nanoprobes Tc-99m- and Gd-usNGO-PEG were reliably prepared with high yields and good stability. Serial comparative experiments of the labeling yield, the measurements of -NH-2 density and zeta-potentials, and various characterizations including energy-dispersive X-ray analysis spectroscopy, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy demonstrated that both usNGO and PEG synergistically provide the electron-donating atoms 0 and N to coordinate with (99m)Tcl and Gd to form stable nanocomplexes. Furthermore, both Tc-99m- and Gd-usNGO-PEG exhibited excellent in vivo imaging of lymph nodes using single -photon emission computed tomography/computed tomography (SPECT/CT) and magnetic resonance (MR) imaging after local injection. Therefore, these results showed the successful establishment of Tc-99m- and Gd-anchored usNGO-PEG using a chelator-free strategy and the potential of multimodality SPECT/CT and MR imaging of lymph nodes.
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