Journal
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Volume 30, Issue -, Pages 95-102Publisher
UNIV CATOLICA DE VALPARAISO
DOI: 10.1016/j.ejbt.2017.10.003
Keywords
Biodiesel; Biomass; Fuels; Fungus; Hydrolysate; Nitrogen; Oleaginous microorganism; Renewable sources; Saccharification; Single cell oil; Sweet sorghum
Categories
Funding
- Bio4Energy
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Background: Dependence on fossil resources, for the production of fuels and energy, has resulted in environmental and financial problems, which require our immediate action in order to reverse the situation. Use of renewable sources for the production of fuels and energy is an important alternative with biodiesel remains as one of the promising options. Aim of this work is to evaluate the fungus Fusarium oxysporum for its potentials to accumulate microbial lipidswhen grown on syntheticmedia and saccharified sweet sorghumstalks. Results: The effect of different carbon sources, nitrogen sources and C/N ratio on the lipid production was initially examined, which resulted in a lipid concentration of 4.4 g/L, with lipid content of 42.6% w/w. Sweet sorghum stalks were able to support growth and lipid production of the fungus, both as carbon source and as nitrogen source. It was also shown that saccharification of the dried stalks is an important step to increase lipid production. Removal of the remaining stalk solids enabled the lipid production during cultivation in increased initial solids of up to 16 w/w. This resulted in a lipid production of 3.81 g/L. Conclusions: It was demonstrated that F. oxysporum can be used as an efficient oleaginous microorganism, with sweet sorghum serving as an excellent raw material for the cultivation of the fungus. The lipids obtained during this work were also found to have a fatty acid profile with good potentials to be used for biodiesel production. (C) 2017 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.
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