Journal
TOXICON
Volume 125, Issue -, Pages 44-49Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2016.11.252
Keywords
H. venatoria venom; Dorsal unpaired median neurons; Voltage-gated sodium channels; Insecticides
Categories
Funding
- National 973 Project of China [2012CB22305]
- National Science Foundation Project the National Science Foundation [31370854, 31570782]
- Science Found for Distinguished Young Scholars of Hunan Province [14E1018]
- Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province [20134486]
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Heteropoda venatoria is a venomous spider species distributed worldwide and has a characteristic habit of feeding on insects. Reverse-phase high-performance liquid chromatography and matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry analyses revealed that H. venatoria venom contains hundreds of peptides with a predominant molecular weights of 3000-5000 Da. Intra-abdominal injection of the venom had severe toxic effects on cockroaches and caused death at higher concentrations. The LD50 was 28.18 mu g/g of body weight in the cockroach. It was found that the venom had potent inhibitory effect on voltage-gated sodium channels (VGSCs) in Periplaneta americana cockroach dorsal unpaired median (DUM) neurons with an IC50 values of 6.25 +/- 0.02 mu g/mL. However, 100 mu g/mL venom only partially blocked VGSC currents in rat dorsal root ganglion cells, a much lower inhibitory effect than that on DUM VGSCs. Our results indicate that the venom of H. venatoria contains diverse neurotoxins that might become new leads for bioinsecticides. (C) 2016 Elsevier Ltd. All rights reserved.
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