Journal
TOXICON
Volume 128, Issue -, Pages 15-22Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2017.01.016
Keywords
Expression; MC-LR; MicroRNA; Silver carp; Toxicity
Categories
Funding
- National Natural Science Foundation of China [31472285]
- Innovation Scientists and Technicians Troop Construction Projects of Henan Province, China [16420050001]
- Key Subjects of Biology and Ecology in Henan Province, China [201604, 201606]
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In the field of toxicology, the relationship between microRNAs (miRNAs) and microcystin-LR (MC-LR) toxicity in fish is still poorly understood. In the present study, we used quantitative real-time PCR (qPCR) to examine the expression of 7 miRNAs (let-7b, miR-21, miR-122, miR-27b, miR-148, miR-125a, and miR143) that play regulatory roles in biological processes such as signal transduction, apoptosis, cell cycle, and fatty acid metabolism. Expression patterns were assessed in the liver, spleen, and kidney of silver carp following 8, 24, and 48 h of exposure to MC-LR via intraperitoneal injection (50 or 200 mu g/kg of body weight). The results of qPCR analysis showed that the expression of the 7 miRNAs was either upregulated or downregulated in response to MC-LR exposure. These results indicate that acute MC-LR exposure altered miRNAs expression profiles in the liver, spleen, and kidney of silver carp, suggesting that miRNAs may be involved in MC-LR toxicity. (C) 2017 Elsevier Ltd. All rights reserved.
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