Journal
BRITISH JOURNAL OF NUTRITION
Volume 118, Issue 11, Pages 959-970Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114517002999
Keywords
Microbiome; Microbiota; n-3 PUFA; SCFA; Metabolomics
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Funding
- Science Foundation Ireland in form of a centre grant (APC Microbiome Institute grant) [SFI/12/RC/2273]
- Health Research Board of Ireland [HRA_POR/2011/23, HRA_POR/2012/32]
- Sea Change Strategy, NutraMara programme [MFFRI/07/01]
- SMART FOOD project: 'Science Based 'Intelligent'/Functional and Medical Foods for Optimum Brain Health, Targeting Depression and Cognition' project [13/F/411]
- Marine Institute
- Department of Agriculture, Food and the Marine in Ireland
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n-3 PUFA are lipids that play crucial roles in immune-regulation, cardio-protection and neurodevelopment. However, little is known about the role that these essential dietary fats play in modulating caecal microbiota composition and the subsequent production of functional metabolites. To investigate this, female C57BL/6 mice were assigned to one of three diets (control (CON), n-3 supplemented (n3+) or n-3 deficient (n3-)) during gestation, following which their male offspring were continued on the same diets for 12 weeks. Caecal content of mothers and offspring were collected for 16S sequencing and metabolic phenotyping. n3- male offspring displayed significantly less % fat mass than n3+ and CON. n-3 Status also induced a number of changes to gut microbiota composition such that n3- offspring had greater abundance of Tenericutes, Anaeroplasma and Coriobacteriaceae. Metabolomics analysis revealed an increase in caecal metabolites involved in energy metabolism in n3+ including alpha-ketoglutaric acid, malic acid and fumaric acid. n3- animals displayed significantly reduced acetate, butyrate and total caecal SCFA production. These results demonstrate that dietary n-3 PUFA regulate gut microbiota homoeostasis whereby n-3 deficiency may induce a state of disturbance. Further studies are warranted to examine whether these microbial and metabolic disturbances are causally related to changes in metabolic health outcomes.
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