A study on the in vitro percutaneous absorption of silver nanoparticles in combination with aluminum chloride, methyl paraben or di-n-butyl phthalate

Some reports indicate that the silver released from dermally applied products containing silver nanoparticles (AgNP) (e.g. wound dressings or cosmetics) can penetrate the skin, particularly if damaged. AgNP were also shown to have cytotoxic and genotoxic activity. In the present study percutaneous absorption of AgNP of two different nominal sizes (Ag15 nm or Ag45 nm by STEM) and surface modification, i.e. citrate or PEG stabilized nanoparticles, in combination with cosmetic ingredients, i.e. aluminum chloride (AlCl3), methyl paraben (MPB), or di-n-butyl phthalate (DBPH) was assessed using in vitro model based on dermatomed pig skin. The inductively coupled plasma mass spectrometry (ICP-MS) measurements after 24 h in receptor fluid indicated low, but detectable silver absorption and no statistically significant differences in the penetration between the 4 types of AgNP studied at 47, 470 or 750 mu g/ml. Similarly, no significant differences were observed for silver penetration when the AgNP were used in combinations with AlCl3 (500 mu M), MPB (1250 mu M) or DBPH (35 mu M). The measured highest amount of Ag that penetrated was 0.45 ng/cm(2) (0.365-0.974 ng/cm(2)) for PEG stabilized Ag15 nm + MPB. (c) 2017 Elsevier B.V. All rights reserved.