Journal
BIOSENSORS & BIOELECTRONICS
Volume 98, Issue -, Pages 305-309Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.07.014
Keywords
Histamine; Immunosensor; Prussian blue-chitosan-gold nanoparticles; Food analysis
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Funding
- National Key Research and Development Program of China [2016YFD0401204]
- Innovation Team Foundation of the Department of Agriculture of Guangdong Province [2016LM2152]
- Guangdong Natural Science Funds for Distinguished Young Scholar [2014A030306026]
- Excellent Young Teachers Program for Higher Education of Guangdong Province [Y920/4026]
- Guangdong Special Support Program [2014TQ01N109]
- National Institutes of Health [CA195607, EB021339]
- Department of Defense Office of Congressionally Directed Medical Research Programs [W81XWH-15-1-0180]
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Histamine (HA) is a biogenic amine that can accumulate to high concentration levels in food as a result of microbial activity and can cause toxic effects in consumers. In this work, a portable electrochemical immunosensor capable of detecting HA with high sensitivity and selectivity was developed. Prussian bluechitosan-gold nanoparticle (PB-CS-AuNP) nanocomposite films with excellent biocompatibility were synthesized and characterized by scanning electron microscopy and energy dispersive X-ray analysis. The PB-CSAuNP were coated onto a screen-printed electrode by one-step electrodeposition and used to conjugate the HA ovalbumin conjugate (HA-Ag). HA was determined by a competition between the coating HA-Ag and the HRP labeled HA antibody (HRP-HA-Ab). After careful optimization of assay conditions and Box-Behnken analysis, the developed immunosensor showed a linear range from 0.01 to 100 mu g/mL for HA in fish samples. The average recoveries from spiked samples ranged from 97.25% to 105%. The biosensor also showed good specificity, reproducibility, and stability, indicating its potential application in monitoring HA in a simple and low cost manner.
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