Journal
BIOSENSORS & BIOELECTRONICS
Volume 98, Issue -, Pages 325-329Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.06.059
Keywords
Bioluminogenic probe; gamma-Glutamyltranspeptidase; Biomarker; Tumor diagnosis
Categories
Funding
- 973 Program [2013CB834505, 2013CB834703]
- National Natural Science Foundation of China [21233011, 91123033, 21273252, 21205122, 21261160488]
Ask authors/readers for more resources
Abnormal expression of gamma-Glutamyltranspeptidase (GGT) in living organisms is closely implicated in the development of several human tumors. The GGT levels in tissue and serum have emerged as a potential criterion for tumor diagnosis. However, precise light up GGT activity in vivo is still challenging due to the signal interferes of background. Bioluminescence based on the firefly luciferase-catalyzed reaction for light production provides a feasible strategy for GGT detection in vivo. In this report, a bioluminogenic probe, Glu-Luc, was designed and synthesized by connecting D-luciferin with gamma-glutamyl group. The cleavage of gamma-glutamyl group is triggered by GGT, resulting in the release of D-luciferin, which generates a bright bioluminescence emission in the present of luciferase and ATP. The probe exhibits very high selectivity and sensitivity toward GGT activity from in vitro to in vivo and in clinical samples, which offers a promising tool for investigations of the GGT-overexpressing related biological process including tumor diagnosis and prognosis in living organisms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available