Journal
BMC GENOMICS
Volume 18, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12864-017-4366-2
Keywords
Citrus sinensis; Blood orange; Navel orange; Fruit quality; Transcriptome; Proteome
Funding
- Innovation enhancement engineering project, department of finance, Sichuan province [2016GXTZ-004]
- Applied and basic research, department of science and technology, Sichuan province [17YYJC0208]
- Research thesis founds, Sichuan Academy of Agricultural Sciences [2017LWJJ-003]
Ask authors/readers for more resources
Background: Transcriptome and proteome analyses on fruit pulp from the blood orange 'Zaohong' and the navel orange ` twenty-first century' were performed to study Citrus sinensis quality-related molecular changes during consecutive developmental periods, including young fruit, fruit-coloring onset and fruit delayed-harvest for two months, during which fruit remained on the trees. Results: The time-course analysis for the fruit developmental periods indicated a complex, dynamic gene expression pattern, with the numbers of differentially expressed genes (DEGs) between the two cultivars being 119, 426 and 904 at the three continuous stages tested during fruit development and ripening. The continuous increase in total soluble solids over the course of fruit development was correlated with up-regulated sucrose phosphate synthase (SPS) transcription levels in both cultivars. Eleven differentially expressed genes between the two cultivars involved in the flavonoid pathway were significantly enriched at the onset of the fruit-coloring stage when anthocyanins were detected in blood orange alone. Among 5185 proteins, 65 up-regulated and 29 down-regulated proteins were co-expressed with their cognate mRNAs with significant transcription and protein expression levels when the fruits from the two cultivars were compared at the fruit delayed-harvest stage. Additionally, important genes participating in the.-aminobutyric acid (GABA) shunt were activated in blood orange at two significant expression levels in the fruit delayed-harvest stage. Thus, organic acids in fruit continuously decreased during this stage. Conclusions: This research was the first to provide a more comprehensive understanding of the differentially expressed genes involved in anthocyanin, sucrose and citrate metabolism at the transcriptome and proteome levels in C. sinensis, especially during the fruit delayed-harvest stage.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available