Journal
CURRENT OPINION IN MICROBIOLOGY
Volume 38, Issue -, Pages 130-136Publisher
CURRENT BIOLOGY LTD
DOI: 10.1016/j.mib.2017.04.006
Keywords
-
Categories
Funding
- Biotechnology and Biosciences Research Council [BB/K003356/1]
- BBSRC [BB/K003356/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/K003356/1] Funding Source: researchfish
Ask authors/readers for more resources
DNA site-specific recombinases are enzymes (often associated with mobile DNA elements) that catalyse breaking and rejoining of DNA strands at specific points, thereby bringing about precise genetic rearrangements. Serine integrases are a group of recombinases derived from bacteriophages. Their unusual properties, including directionality of recombination and simple site requirements, are leading to their development as efficient, versatile tools for applications in experimental biology, biotechnology, synthetic biology and gene therapy. This article summarizes our current knowledge of serine integrase structure and mechanism, then outlines key factors that affect the performance of these phage recombination systems. Recently published studies, that have expanded the repertoire of available systems and reveal system-specific characteristics, will help us to choose the best integrases for envisaged applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available