Journal
CANCER BIOMARKERS
Volume 20, Issue 4, Pages 425-434Publisher
IOS PRESS
DOI: 10.3233/CBM-170188
Keywords
Bladder cancer; lncRNA HULC; cell proliferation; cell apoptosis; ZIC2; PI3K/AKT
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BACKGROUND: Bladder cancer is the fourth most common malignancy among men urinary system and it is a complex disease caused by genetic and environmental factors. OBJECTIVE: This study aimed to evaluate the effects of hepatocellular carcinoma up-regulated long non-coding RNA ( lncRNA HULC) on bladder cancer and to reveal the potential mechanisms. METHODS: The expression level of HULC in 276 bladder cancer patients was detected. The association of HULC level with patient recurrence was performed by Kaplan-Meier and log-rank test. Moreover, T24 and RT4 cells were transfected with HULC and ZIC2 targeted siRNAs, HULC expressing vector and corresponding controls. Subsequently, cell viability, apoptosis and tumorigenesis were examined. RESULTS: The expression level of HULC was increased in bladder cancer tissues. High expression of HULC was correlated with advanced clinical stage and lower recurrence-free rate. HULC was remarkably promoted cell viability but inhibited apoptosis, meanwhile conspicuously increased the expression of Cyclin A/D1/E and Bcl-2. Xenograft tumor model showed that HULC promoted tumor weights in vivo. CONCLUSIONS: LncRNA HULC promoted bladder cancer cells proliferation and inhibited apoptosis.
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