Journal
ADVANCES IN COLLOID AND INTERFACE SCIENCE
Volume 250, Issue -, Pages 40-53Publisher
ELSEVIER
DOI: 10.1016/j.cis.2017.10.006
Keywords
Chain flexibility; Electrostatic interactions; Surface patch binding; Complex coacervation; Biomolecules
Categories
Funding
- University Grants Commission (UGC-BSR), Government of India
- DST-PURSE-II [PAC-JNU-DST-PURSE-462]
- Department of Science and Technology, Government of India-Inspire Faculty Award
- Department of Science and Technology, Government of India (DST-INSPIRE) [EMR/2015/001827, EMR/2016/004868]
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In this review, a number of systems are described to demonstrate the effect of polyelectrolyte chain stiffness (persistence length) on the coacervation phenomena, after we briefly review the field. We consider two specific types of complexation/coacervation: in the first type, DNA is used as a fixed substrate binding to flexible polyions such as gelatin A, bovine serum albumin and chitosan (large persistence length polyelectrolyte binding to low persistence length biopolymer), and in the second case, different substrates such as gelatin A, bovine serum albumin, and chitosan were made to bind to a polyion gelatin B (low persistence length substrate binding to comparable persistence length polyion). Polyelectrolyte chain flexibility was found to have remarkable effect on the polyelectrolyte-protein complex coacervation. The competitive interplay of electrostatic versus surface patch binding (SPB) leading to associative interaction followed by complex coacervation between these biopolymers is elucidated. We modelled the SPB interaction in terms of linear combination of attractive and repulsive Coulombic forces with respect to the solution ionic strength. The aforesaid interactions were established via a universal phase diagram, considering the persistence length of polyion as the sole independent variable. (C) 2017 Elsevier B.V. All rights reserved.
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