Journal
TETRAHEDRON
Volume 73, Issue 3, Pages 272-277Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tet.2016.12.012
Keywords
Fluorogenic probe; Coumarin; Thiols; Glutathione; Cell imaging
Categories
Funding
- National Natural Science Foundation [21371148]
- Key Scientific and Technological Project of Henan Province [122102310196]
- Education Project of Henan Province [12A150019]
- Disciplinary group of Psychology and Neuroscience, Xinxiang Medical University
Ask authors/readers for more resources
Glutathione is the most abundant non-protein thiols compound in cells and plays important metabolic roles. Changes in the amount of glutathione or its metabolic dysregulation can lead to a series of diseases. The determination of glutathione levels is very helpful to the diagnosis and treatment of the related diseases. A coumarin schiff base (compound 1) was synthesized from coumarin hydrazide and 2,6-pyridine dicarboxaldehyde and the fluorogenic probe for thiols (compound 1-Cu2+) was prepared by coordinating compound 1 with copper ions. Compound 1 showed strong fluorescence, while compound 1-Cu2+ hardly had fluorescence due to the paramagnetism and/or photoinduced electron transfer of Cu2+. However, after the addition of thiols-containing compounds, the fluorescence of compound 1 was restored. The UV vis absorption and fluorescence spectra indicated that the fluorogenic probe had good thiols selectivity and sensitivity, particularly for glutathione in CH3CN:HEPES (3:2, v/v) buffer. It was successfully applied to the fluorescence imaging detection of glutathione in human cervical squamous cancer cells (SiHa cells). (C) 2016 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available