Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 44, Issue 3, Pages 998-1010Publisher
KARGER
DOI: 10.1159/000485400
Keywords
Osterix; GALNT14; Chemosensitivity; Breast cancer
Categories
Funding
- National Natural Science Foundation of China [81372320, 81570804, 81502303]
- Natural Science Foundation of Jiangsu Educational Committee [15KJB310006]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Key Project of Science and Technology Development Foundation of Nanjing Medical University [2014NJMUZD009]
- Jiangsu Higher College Students' Innovative Entrepreneurial Training Program [201510312019Z]
Ask authors/readers for more resources
Background/Aims: Osterix (Osx), a key regulator of osteoblast differentiation and bone formation, has been recently reported to be associated with the progression of breast cancer. However, the precise roles of Osx in breast cancer remain unclear. Methods: Drug sensitivity of the cancer cells was assessed using an 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide (MTT) assay. Target genes were obtained by high-throughput Illumina sequencing and were confirmed in vitro and in vivo. Apoptosis was analysed by Hoechst staining and western blotting. A tissue microarray including 129 samples from breast cancer patients was used for immunohistochemistry (IHC) assays. Results: Overexpression of Osx decreased the chemosensitivity of breast cancer cells, while knockdown of Osx increased the chemosensitivity of breast cancer cells. In particular, we found that the decreased chemosensitivity effect was significantly associated with elevated expression of the polypeptide N-acetylgalactosaminyltransferase 14 (GALNT14). Silencing of GALNT14 in Osx-overexpressed cells restored the decreased chemosensitivity. Conversely, overexpression of GALNT14 in Osx-knockdown cells abrogated the increased chemosensitivity in breast cancer cells. In addition, we revealed that Osx decreased GALNT14-dependent chemosensitivity by enhancing antiapoptosis. GALNT14 expression exhibited a significant association with breast cancer stages as well as the disease-free survival (DFS) rate. Conclusion: Osx plays an important role in the chemosensitivity and inhibition of Osx expression may represent a therapeutic strategy to enhance the chemosensitivity of breast cancer. (C) 2017 The Author(s) Published by S. Karger AG, Basel.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available