Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 23, Issue 69, Pages 17612-17620Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201704563
Keywords
DNA; minor groove binder; mixed base pair recognition; NMR spectroscopy; solution structures
Categories
Funding
- National Institutes of Health [NIH R01 GM111749]
- Molecular Basis of Disease Fellowship
Ask authors/readers for more resources
The high-resolution NMR structure of the first heterocyclic, non-amide, organic cation that strongly and selectively recognizes mixed AT/GC bp (bp=base pair) sequences of DNA in a 1:1 complex is described. Compound designs of this type provide essential methods for control of functional, non-genomic DNA sequences and have broad cell uptake capability, based on studies from animals to humans. The high-resolution structural studies described in this report are essential for understanding the molecular basis for the sequence-specific binding as well as for new ideas for additional compound designs for sequence-specific recognition. The molecular features, in this report, explain the mechanism of recognition of both AT and GC bps and are an interesting molecular recognition story. Examination of the experimental structure and the NMR restrained molecular dynamics model suggests that recognition of the GC base pair involves two specific H-bonds. The structure illustrates a wealth of information on different DNA interactions and illustrates an interfacial water molecule that is a key component of the complex.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available