Journal
TALANTA
Volume 174, Issue -, Pages 521-526Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2017.06.048
Keywords
Surface plasmon resonance; Graphene oxide-gold nanoparticles composites; MicroRNA; Adenosine
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Funding
- National Natural Science Foundation of China [21375034, 21675047, 21190040]
- Natural Science Foundation for Distinguished Young Scholars of Hunan Province [2016JJ1008]
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A versatile and sensitive surface plasmon resonance (SPR) biosensor based on two layers of graphene oxide-gold nanoparticles (GO-AuNPs) composites was designed for the detection of microRNA (miRNA) and small molecule adenosine. The bottom layer, which acted as a functionalized substrate on the sensor chip, provided a high specific surface area convenient for the immobilization of capture DNA molecules. The upper layer served as a signal-amplification element. By employing these two layers of GO-AuNPs composites, the dual amplification strategy was achieved so that a measurement of miRNA-141 with a detection limit of 0.1 fM was obtained. Moreover, the developed SPR biosensor showed decent selectivity toward miRNA-200 family members. Especially, the SPR biosensor demonstrated its applicability for the detection of miRNA-141 in cancer cell extractions, and the results obtained were consistent with those obtained by qRT-PCR. Interestingly, small molecule adenosine could also be detected using this SPR biosensor in combination with a split aptamer. Considering the superior sensitivity, selectivity and generality, this work promised much potential for the detection of various biomolecules.
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