Journal
TALANTA
Volume 166, Issue -, Pages 187-192Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2017.01.053
Keywords
Microcystin-LR; Fluorescent aptasensor; Dapoxyl; Single-walled carbon nanotubes
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Funding
- Mashhad University of Medical Sciences
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To assure water safety and protect human health, precise and simple analytical approaches are highly desired to determine low concentrations of microcystin-leucine-arginine (MC-LR), a toxin, in both water and serum samples. Herein, a simple, rapid and accurate aptamer-based fluorescent sensor was used for selective recognition of MC-LR, based on single-walled carbon nanotubes (SWNTs) as immobilizers, dapoxyl as a fluorescent dye, DAP-10 as a specific aptamer for dapoxyl and unmodified MC-LR aptamer (Apt) as a sensing ligand. The sensing method was developed to produce a remarkable fluorescence intensity difference in the absence and presence of MC-LR. Moreover, the Apt was used without any modification. In the absence of MC-LR, the dapoxyl could bind to DAP-10, leading to a strong fluorescence intensity. In the presence of MC-LR, DAP-10 bound to the surface of SWNTs, resulting in a very weak fluorescence intensity. Under optimized conditions, the presented fluorescent analytical approach showed high selectivity toward MC-LR with a limit of detection (LOD) of 138 pM. This new method indicated excellent analytical performance for MC-LR detection in tap water and serum samples with LODs of 135 and 168 pM, respectively.
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