Journal
TALANTA
Volume 162, Issue -, Pages 46-51Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2016.09.066
Keywords
Graphite carbon nitride; Fluorescent biosensor; Aptamer; Mercuric ion
Categories
Funding
- National Natural Science Foundation of China [21375047, 21377046, 21575050, 21505051]
- Science and Technology Plan Project of Jinan [201307010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- Special Project for Independent Innovation and Achievements Transformation of Shandong Province [2014ZZCX05101]
- Graduate Innovation Foundation of University of Jinan (GIFUJN)
- Special Foundation for Taishan Scholar Professorship of Shandong Province [ts20130937]
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A novel and simple fluorescent biosensor has been realized through the fluorescence quenching of graphite carbon nitride (g-C3N4) for mercuric ion (Hg2+) detection. In this assay, the g-C3N4 sheets which were functionalized with single-stranded DNA (ssDNA) aptamer showed strong fluorescence emission at 440 nm under the excitation of 380 nm in the absence of Hg2+. When added to the assay solution, Hg2+ was embedded in the hairpin-shaped double-stranded DNA (dsDNA) due to the formation of the thymine-Hg2+-thymine (T-Hg2+-T) complex, which made the Hg2+ close to the surface of g-C3N4 sheet. Therefore, the fluorescence of g-C3N4 was quenched. This sensor has good selectivity with a limit of detection as low as 0.17 nM under optimal conditions. The present work demonstrates that the g-C3N4-based fluorescent sensor has a promising application for detection of metal ions in real samples.
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