Journal
TALANTA
Volume 165, Issue -, Pages 593-597Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2017.01.028
Keywords
Fluorescent probe; DCM; Lactulose; Peroxynitrite; Full aqueous
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Funding
- Science and Technology Plan Project in Zhejiang Province [2015C33224]
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A selective fluorogenic boronate-based probe-lactulose complex was evaluated for the rapid analysis of peroxynitrite (ONOO-) based on a reaction-based indicator displacement assay (RIA). The probe was synthesised by a simple nucleophilic substitution reaction between a boronic acid moiety and a well known laser dye, DCM. Fluorescence analyses showed that the probe had an off-on response to lactulose, forming a fluorogenic probe-lactulose complex. The subsequent addition of ONOO- selectively quenched the fluorescence of the complex over other Reactive Oxygen/ Nitrogen Species (ROS/RNS) tested. The complex can be applied for the rapid determination of ONOO- in full aqueous solution with good linear range, and has also proven suitable for monitoring ONOO- in living cells and real water samples.
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