Journal
TALANTA
Volume 164, Issue -, Pages 580-587Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2016.10.008
Keywords
Upconversion nanoparticles; Inner ffiter effect; H2O2; Glucose; SQA-iron(III)
Categories
Funding
- National Natural Science Foundation of China [21375037, 21275051, 21475043]
- Scientific Research Fund of Hunan Provincial Science and Technology Department
- Education Department [13JJ2020, 12A084]
- Ministry of Education of China [20134306110006]
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Diabetes mellitus is an epidemic disease that it has became a worldwide public health problem. Thus, blood glucose monitoring has attracted extensive attention. Here, we report a nanosensor based on inner filter effect (IFE) between upconversion nanoparticles (UCNPs) and squaric acid (SQA)-iron(III) for the highly sensitive and selective detection of glucose levels in human serum. In this assay, GOx-catalyzed oxidization of glucose produces gluconic acid and hydrogen peroxide (H2O2). The latter can catalytically oxidize iron(II) to iron(III) which can rapidly (< 1 min) coordinate with the SQA to produce (SQA)-iron(III). The absorption band of (SQA)-iron(III) largely covered the emission band of UCNPs, resulting the fluorescence emission of UCNPs was effectively quenched. Therefore, the glucose can be monitored based on the formation of SQA-iron(III). Under the optimal condition, the fluorescence quenching efficiency shows a good linear response to glucose concentration in the ranges of 7-340 mu mol/L with a detection limit of 2.3 mu mol/L. The developed method has been further applied to monitor glucose levels in human serum with satisfactory results. Compared with other fluorescence methods, current method displayed high sensitivity and signal-to-noise ratio. Meanwhile, this nanosystem is also generalizable and can be easily expanded to the detection of various H2O2-involved analytes.
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