4.7 Article

A simple and fast chromogenic reaction based on Ag3PO4/Ag nanocomposite for tumor marker detection

Journal

TALANTA
Volume 175, Issue -, Pages 229-234

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2017.07.039

Keywords

Colorimetric; Immunoassay; Ag3PO4/Ag, Hydrolysis

Funding

  1. National Natural Science Foundation of China [21605111]
  2. Natural Science Foundation of Shanxi Province [201601D021037]
  3. Youth Foundation of Taiyuan University of Technology [2015QN033]
  4. Shanxi Scholarship Council of China [2015-042]

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Colorimetric assay is a powerful tool for detection of tumor markers with the outstanding advantages of visualization and simple analytical instruments. Herein, a simple and fast chromogenic reaction was developed based on Ag3PO4/Ag nanocomposite synthesized by one-step chemical bath method. And the Ag3PO4/Ag nanocomposite owned the ability of oxidizing of TMB under the acidic condition due to the hydrolytic action. The nanocomposite was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), X-ray powder diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). Then, the colorimetric immunoassay was constructed by utilizing Ag3PO4/Ag nanocomposite as molecular labels for carcino embryonie antigen (CEA, UniProt accession number: P06731) detection. The blue color with gradient change was observed with the increasing of CEA concentrations. Under optimal conditions, the variation between absorbance values and CEA levels was linear in the range of 0.2 ng mL(-1) similar to 2.5 ng mL(-1) with the detection limit of 0.03 ng mL(-1). More importantly, the developed colorimetric immunoassay exhibited good selectivity, repeatability, stability and potential application in the real serum sample detection.

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