Journal
TALANTA
Volume 170, Issue -, Pages 399-405Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2017.04.019
Keywords
Fenton-type reaction; Poly(thionine); Electroreduction; Inhibition action; Reduced glutathione
Categories
Funding
- Scientific Research Fund of Hunan Provincial Education Department [14A095]
- Open Sustentation Fund of State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University [2014007]
Ask authors/readers for more resources
An indirect and sensitive electrochemical method for the determination of reduced glutathione (GSH) was developed by using poly(thionine) (PTH) as a mediator in the presence of Fenton-type reaction on the electrode surface in this work. Cyclic voltammetry and the adsorption of Cu2+ were employed in sequence to fabricate a Cu-PTH modified electrode, which was characterized by scanning electron microscopy, XPS and electrochemical measurements. Hydroxyl radicals that were derived from the Fenton-type reaction between Cu2+ and H2O2 could effectively oxidize PTH, leading to the great enhancement of the cathodic peak current of the dye polymer in the cyclic voltammetric scan. The electroreduction of PTH on the electrode surface was found to be inhibited in the presence of GSH. Under the optimized conditions, the cathodic peak current change was found to be proportional to the logarithm of the GSH concentration from 10 nM to 1 mM with a detection limit of 2.5 nM estimated at a signal-to-noise ratio of 3. The proposed electrochemical sensor was successfully employed for the determination of GSH in real samples with satisfactory results.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available