Journal
TALANTA
Volume 171, Issue -, Pages 53-60Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2017.04.058
Keywords
Sequential injection; Amperometry; Immunosensor; Graphene oxide; Human immunoglobulin G; Urine
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Funding
- Science Achievement Scholarship of Thailand (SAST)
- National Research University Project under Thailand's Office of the Higher Education Commission, Center of Excellence for Innovation in Chemistry
- Graduate School and Department of Chemistry, Faculty of Science, Chiang Mai University
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Sequential injection (SI) system incorporated with amperometric immunosensor was developed for sensitive determination of human immunoglobulin G (HIgG). A cost effective label-free immunosensor was fabricated by immobilizing anti-HIgG on a graphene oxide (GO) modified screen-printed carbon electrode (SPCE). The developed electrode was characterized by cyclic voltammetry(CV), scanning electron microscope(SEM), and energy dispersive spectroscopy(EDS) which confirmed the selective immunointeraction of HIgG to the anti-HIgG on the electrode, thus reduced the amperometric current of [Fe(CN)(6)](3-/4-) redox probe. The sensing electrode was placed in a designed electrochemical flow cell of SI system, where the redox probe was propelled through and the currents before and after the immunointeraction occurred were measured amperometrically by using a simple home-made amperometer. Under the optimum condition: flow rate of 2 mL min(-1), applied potential of +350 my, [Fe(CN)(6)](3-/4-) concentration of 10 mM and 10 min of incubation time, a linear calibration in the range of 2-100 ng mL(-1) was achieved, with detection limit of 1.70 ng mL(-1). The proposed system provided good repeatability and reproducibility and the application for urine sample analysis was demonstrated.
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