Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 40, Issue 7, Pages 448-457Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.syapm.2017.07.004
Keywords
Anammox enrichment culture; 'Ca. Brocadia sapporoensis'; Physiological characteristics; Comparative genome analysis
Categories
Funding
- Nagase Science and Technology Foundation
- Institute for Fermentation, Osaka (IFO)
- Monbukagakusho Honors Scholarship
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
- Graduate school of Engineering, Hokkaido University, Japan
Ask authors/readers for more resources
We successfully enriched a novel anaerobic ammonium-oxidizing (anammox) bacterium affiliated with the genus 'Candidatus Brocadia' with high purity (>90%) in a membrane bioreactor (MBR). The enriched bacterium was distantly related to the hitherto characterized 'Ca. Brocadia fulgida' and 'Ca. Brocadia sinica' with 96% and 93% of 16S ribosomal RNA gene sequence identity, respectively. The bacterium exhibited the common structural features of anammox bacteria and produced hydrazine in the presence of hydroxylamine under anoxic conditions. The temperature range of anammox activity was 20-45 degrees C with a maximum activity at 37 degrees C. The maximum specific growth rate (mu(max).) was 0.0082 h(-1) at 37 degrees C, corresponding to a doubling time of 3.5 days. The half-saturation constant (K-s) for nitrite was 5 +/- 2.5 mu M. The anammox activity was inhibited by nitrite (IC50 = 11.6 mM) but not by formate and acetate. The major respiratory quinone was identified to be menaquinone-7 (MK-7). The enriched anammox bacterium shared nearly half of genes with 'Ca. Brocadia sinica' and 'Ca. Brocadia fulgida'. The enriched bacterium showed all known physiological characteristics of anammox bacteria and can be distinguished from the close relatives by its 16S rRNA gene sequence. Therefore, we proposed the name 'Ca. Brocadia sapporoensis' sp. nov. (C) 2017 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available