Journal
8TH INTERNATIONAL CONFERENCE ON GLOBAL RESOURCE CONSERVATION (ICGRC 2017): GREEN CAMPUS MOVEMENT FOR GLOBAL CONSERVATION
Volume 1908, Issue -, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.5012721
Keywords
auxin; cytokinin; in vitro culture; callus formation; Physalis angulata L.; medicinal plant
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Funding
- Directorate of Research and Community Service, Directorate General for Research and Development at the Ministry of Research, Technology and higher Education [063/SP2H/LT/DRPM/IV/2017]
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Physalis angulata L. (Ciplukan) is one member of Solanaceae that has a potential as herbal medicine. This plant grows wild in the crop fields, forest edges, etc. However, ciplukan is increasingly difficult to find recently. In vitro callus is an alternative source to produce secondary metabolite production as well as to regenerate plants through indirect organogenesis. This study aims to identify the response of hypocotyl explants on in vitro callus formation induced by a combination of auxin and cytokinins. Two types of cytokinins, Kinetin and BAP (0.5 ppm) were combined with three types of auxin, i.e. 2.4-D, IBA and IAA, at three concentrations 0.5, 1.0 and 1.5 ppm. In all combinations of cytokinin and auxin, 50-100% of hypocotyl explants derived from in vitro seedling were able to produce callus either in a compact or watery friable texture. In MS medium supplemented with 2.4-D, callus LW (fresh weight) began to decline in the fourth week after culture. Callus FW that increased until 5 weeks of culture was obtained in medium IAA 0.5 Kin 0.5, IBA 1.0 Kin 0.5 and IBA 1 BA 0.5. Almost all calli induced on a medium Kinetin also produced roots. While medium + BAP was able to induce shoots regeneration.
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