3.8 Article

Inhibitory Potential Of Lactobacillus Plantarum on Escherichia Coli

Publisher

UNIV AGRICULTURAL SCIENCES & VETERINARY MEDICINE CLUJ-NAPOCA
DOI: 10.15835/buasvmcn-fst:0031

Keywords

E. coli; inhibitory potential; L. plantarum; pathogenic strains; probiotics

Funding

  1. UEFISCDI [PN-II-PT-PCCA-2013-4-0743]

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An alternative therapy to diarrhoeal episodes induced by E. coli is represented by probiotic strains (e.g Lactobacillus), which are able to maintain the gut micro-flora in optimal parameters. This work highlights the inhibitory potential of probiotics (L. plantarum ATCC 8014) over the enteropathogenic bacteria like E. coli ATCC 25922. For this study we co-incubated L. plantarum with E. coli in MRS broth at different concentrations (10(9), 10(8) CFU/mL - L. plantarum with 10(5), 10(4) CFU/mL - E. coli, 4 groups) for 24, 48 and 72h at 37 degrees C. After co-incubation, E. coli colonies developed on Levine media were counted towards the control. E. coli colonies decreased after 48 and 72h of co-incubation with L. plantarum. Also, was noticed that L. plantarum (10(8) CFU/mL) completely inhibits E. coli (10(4) CFU/mL) after 72h of co-incubation. This work proves the inhibitory potential of probiotic strains (L. plantarum ATCC 8014) against enteropathogenic bacteria (E. coli ATCC 25922).

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