Journal
STRUCTURE
Volume 25, Issue 7, Pages 967-+Publisher
CELL PRESS
DOI: 10.1016/j.str.2017.05.003
Keywords
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Funding
- NIH [CA098468, CA207416, T32 DK007737, GM102137, HL094463]
- Eshelman Institute for Innovation
- Tom and Elizabeth Long Research Award
- J. Thurman Frieze Summer Research Scholarship
- University of North Carolina [5T32GM008570-20]
- NSF
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Microbiome-encoded beta-glucuronidase (GUS) enzymes play important roles in human health by metabolizing drugs in the gastrointestinal (GI) tract. The numbers, types, and diversity of these proteins in the human GI microbiome, however, remain undefined. We present an atlas of GUS enzymes comprehensive for the Human Microbiome Project GI database. We identify 3,013 total and 279 unique microbiome-encoded GUS proteins clustered into six unique structural categories. We assign their taxonomy, assess cellular localization, reveal the inter-individual variability within the 139 individuals sampled, and discover 112 novel microbial GUS enzymes. A representative in vitro panel of the most common GUS proteins by read abundances high-lights structural and functional variabilities within the family, including their differential processing of smaller glucuronides and larger carbohydrates. These data provide a sequencing-to-molecular roadmap for examining microbiome-encoded enzymes essential to human health.
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