Journal
STRUCTURE
Volume 25, Issue 1, Pages 79-93Publisher
CELL PRESS
DOI: 10.1016/j.str.2016.11.008
Keywords
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Funding
- Diamond Light Source [I03, I24, B21]
- Austrian Science Fund (FWF) [I24] Funding Source: Austrian Science Fund (FWF)
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Streptococcus pneumoniae is dependent on carbohydrate uptake for colonization and pathogenesis, and dedicates over a third of its transport systems to their uptake. The ability of the pneumococcus to utilize fructooligosaccharides (FOSs) is attributed to the presence of one of two types of FOS ATP-binding cassette (ABC) transporters. Strains encoding SfuABC are only able to utilize short-chain FOSs, while strains encoding FusABC can utilize both short-and long-chain FOSs. The crystal structures of the substrate-binding protein FusA in its open and closed conformations bound to FOSs, and solution scattering data of SfuA, delineate the structural basis for import of short-and long-chain FOSs. The structure of FusA identifies an EF hand-like calcium-binding motif. This is shown to be essential for translocation of FOSs in FusABC and forms the basis for the definition of a new class of substratebinding proteins that regulate substrate translocation by calcium.
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