Journal
STRUCTURE
Volume 25, Issue 4, Pages 663-+Publisher
CELL PRESS
DOI: 10.1016/j.str.2017.02.005
Keywords
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Funding
- HHMI
- NIH [R01 GM55440]
- China Scholarship Council [201406120114]
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We describe a spraying-plunging method for preparing cryoelectron microscopy (cryo-EM) grids with vitreous ice of controllable, highly consistent thickness using a microfluidic device. The new polydimethylsiloxane (PDMS)-based sprayer was tested with apoferritin. We demonstrate that the structure can be solved to high resolution with this method of sample preparation. Besides replacing the conventional pipetting-blotting-plunging method, one of many potential applications of the new sprayer is in time-resolved cryo-EM, as part of a PDMS-based microfluidic reaction channel to study short-lived intermediates on the timescale of 10-1,000 ms.
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