Synthesis and spectroscopic studies of Ru(II) complexes of steroidal thiosemicarbazones by multi step reaction: As anti-bacterial agents

Ru(II) steroidal metal complexes were synthesized by the reaction of dichlorodicarbonyl ruthenium(II) [Ru (CO)(2)Cl-2](n) with Steroidal thiosemicarbazones. Coordination via the thionic sulfur and the azomethine nitrogen atom of the thiosemicarbazone to the Ru(II) metal. Steroidal thiosemicarbazone derivatives were obtained by the thiosemicarbazide with steroidal ketones. Structures of the steroidal thiosemicarbazone and their metal complexes were confirmed by the FT-IR, H-1 NMR, C-13 NMR, Fab-Mass spectroscopy and elemental analysis. The antibacterial activity of these compounds were first tested in vitro by the disk diffusion assay against two Grampositive and two Gram-negative bacteria, and then the minimum inhibitory concentration (MIC) was determined. The results showed that steroidal Ru(II) complexes are better inhibit growth as compared to steroidal thiosemicarbazones of both types of the bacteria (gram-positive and gram-negative).