Journal
STEM CELL RESEARCH
Volume 25, Issue -, Pages 256-265Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scr.2017.10.015
Keywords
Human iPS cells; TALEN; Knock-in technology; p63; Stem cells; Epithelial stem cells; Corneal epithelium
Funding
- Realization of Regenerative Medicine of The Japan Agency for Medical Research and Development (AMED)
- BBSRC [BB/M025349/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M025349/1] Funding Source: researchfish
- Grants-in-Aid for Scientific Research [25253093] Funding Source: KAKEN
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The expression of p63 in surface ectodermal cells during development of the cornea, skin, oral mucosa and olfactory placodes is integral to the process of cellular self-renewal and the maintenance of the epithelial stem cell status. Here, we used TALEN technology to generate a p63 knock-in (KI) human induced pluripotent stem (hiPS) cell line in which p63 expression can be visualized via enhanced green fluorescent protein (EGFP) expression. The KI-hiPS cells maintained pluripotency and expressed the stem cell marker gene,Delta Np63 alpha. They were also able to successfully differentiate into functional corneal epithelial cells as assessed by p63 expression in reconstructed corneal epithelium. This approach enables the tracing of p63-expressing cell lineages throughout epithelial development, and represents a promising application in the field of stem cell research. (C) 2017 The Authors. Published by Elsevier B.V.
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