Journal
STEM CELL RESEARCH
Volume 18, Issue -, Pages 54-56Publisher
ELSEVIER
DOI: 10.1016/j.scr.2016.12.010
Keywords
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Funding
- National Medical Research Council (NMRC) of Singapore
- Translational Clinical Research (TCR) award [NMRC/TCR/013-NNI/2014]
- STaR award [NMRC/STaR/014/2013]
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Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 64-year old male Parkinson's disease (PD) patient with S1647T variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model will be useful for further function studies and therapeutic screening. (C) 2016 The Authors. Published by Elsevier B.V.
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