Journal
CHINESE CHEMICAL LETTERS
Volume 28, Issue 10, Pages 2019-2022Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2017.07.030
Keywords
DNA damage; Ratiometric sensor; Fluorescenct imaging; Nucleus labeling; Naphthalimide
Categories
Funding
- National Natural Science Foundation of China [21376038, 21421005, 21576040]
- National Basic Research Program of China [2013CB733702]
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A ratiometric fluorescent sensor (Hoe-NI) was developed by connecting a nucleus targeted Hoechst unit to a naphthalimide dye via click chemistry. The sensor achieves high specific nucleus labeling with wash-free staining method in various kinds of living cells. The fluorescence ratio of the two emission bands (450 nm for Hoechst and 505 nm for naphthalimide) is changed sensitively to the variation of DNA concentrations, which provides the quantitative information in the processes of DNA damage induced by hydroxyl radicals and antitumor drug. Therefore, Hoe-NI is a recommendable sensor for the monitoring of nuclear DNA damage that reveals the health status of cells. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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